可“嗅到光线”的老鼠有助于深入了解嗅觉

Harvard University neurobiologists have created mice that can "smell" light, providing a potent¹（有效的，强有力的） new tool that could help researchers better understand the neural² basis of olfaction（嗅觉） . The work, described this week in the journal Nature Neuroscience, has implications for the future study of smell and of complex perception systems that do not lend themselves to easy study with traditional methods.

"It makes intuitive（直觉的） sense to use odors（气味，名声） to study smell," says Venkatesh N. Murthy, professor of molecular³ and cellular⁴ biology at Harvard. "However, odors are so chemically complex that it is extremely difficult to isolate⁵ the neural circuits underlying⁶ smell that way."

Murthy and his colleagues at Harvard and Cold Spring Harbor Laboratory used light instead, applying the infant field of optogenetics（光遗传学） to the question of how cells in the brain differentiate⁷ between odors.

Optogenetic techniques integrate light-reactive proteins into systems that usually sense inputs⁹ other than light. Murthy and his colleagues integrated these proteins, called channelrhodopsins, into the olfactory¹⁰ systems of mice, creating animals in which smell pathways were activated¹¹ not by odors, but rather by light.

"In order to tease（取笑，戏弄） apart how the brain perceives differences in odors, it seemed most reasonable to look at the patterns of activation¹² in the brain," Murthy says. "But it is hard to trace these patterns using olfactory stimuli¹³, since odors are very diverse and often quite subtle（微妙的，精细的） . So we asked: What if we make the nose act like a retina（视网膜） ?"

With the optogenetically engineered animal, the scientists were able to characterize the patterns of activation in the olfactory bulb, the brain region that receives information directly from the nose. Because light input⁸ can easily be controlled, they were able to design a series of experiments stimulating¹⁴ specific sensory¹⁵ neurons in the nose and looking at the patterns of activation downstream in the olfactory bulb.

"The first question was how the processing is organized, and how similar inputs are processed by adjacent（临近的） cells in the brain," Murthy says.

But it turns out that the spatial¹⁶ organization（空间结构） of olfactory information in the brain does not fully¹⁷ explain our ability to sense odors. The temporal organization of olfactory information sheds additional light on how we perceive odors. In addition to characterizing the spatial organization of the olfactory bulb, the new study shows how the timing¹⁸ of the "sniff¹⁹" plays a large part in how odors are perceived.

The paper has implications not only for future study of the olfactory system, but more generally for teasing out the underlying neural circuits of other systems.