新方法分离仙人掌DNA

Isolation¹ of DNA² from some organisms is a routine procedure. For example, you can buy a kit³ at your local pharmacy⁴ or grocery store that allows you to swab（打扫，擦拭） the inside of your cheek and send the sample for DNA sequencing. However, for other organisms, DNA extraction is much more problematic. Researchers at Desert Botanical Garden in Phoenix⁵, Arizona, have developed a novel procedure that greatly simplifies genomic DNA isolation from cactus⁶（仙人掌） tissue. For members of the family Cactaceae, isolation of genetic⁷ material can be difficult due to the presence of polysaccharide（多糖）-based mucilage content and other secondary compounds. Although important for water storage, these compounds necessitate⁸ the use of toxic⁹ chemicals and numerous modifications¹⁰ to protocols¹¹ for DNA extraction. Lead author Shannon D. Fehlberg and colleagues describe a novel method for isolation of DNA using cactus spines¹³ in the March issue of Applications in Plant Sciences.

 

"I had worked with getting DNA out of cactus in the past where you use pieces of the epidermis¹⁴（上皮，表皮）, but it was messy and difficult to sample. It was also difficult to deal with in the lab because of the mucilage（粘液，胶水）," says Fehlberg. "Now you can snip¹⁵ a spine¹² and, while you have to grind the spine up, it is easy to collect and easy to store and you can follow the manufacturer's protocols for extraction -- simplifying both the field and genetic work."

 

Considered to be modified leaves, spines contain significantly less mucilage content compared to other tissues commonly used for sampling in Cactaceae. Additionally, removal of cactus spines is less invasive than sampling epidermal¹⁶ tissue, which can damage plants and expose the underlying¹⁷ soft tissue to pathogens.

 

"Although you can cut a fairly small sample of epidermal tissue, this can be problematic if you are working with living collections or endangered species. Not only is it much easier to clip a spine, it is also more aesthetic¹⁸ and less harmful," comments Fehlberg.

 

As the cost of DNA sequencing has dramatically decreased, its use has grown exponentially. Because it allows the comparison of individuals within and between populations, DNA sequencing has played an important role in understanding genetic diversity. "For example, in the plant species I'm studying, the species boundaries are not clear," says Fehlberg. "Genetics is important for determining what can be considered a cohesive¹⁹ group. "

 

Knowledge of genetic variation among populations will provide insight to the persistence²⁰ of a species and inform conservation efforts. Fehlberg notes, "Genetics is helpful in determining how similar populations are to one another and how connected they are. We're able to use both genetics and biological information to determine which populations are most unique and which are most threatened."

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